ACTA BIOLOGICA CRACOVIENSIA Series Botanica 47/2: 77-82, 2005

RAPD Analysis of Genetic Relations Between Büzgülü Grape Cultivars 
(Vitis vinifera) Grown in Different Parts of Turkey

Sümer Aras1*, Janset Burcin Polat1, Demet Cansaran1, and Gökhan Söylemezoglu2 

1Department of Biology, University of Ankara, 06100, Turkey
2Department of Horticulture, Faculty of Agriculture, University of Ankara, 06110, Turkey

*e-mail: aras@science.ankara.edu.tr

Received March 7, 2005; revision accepted June 28, 2005

The genetic relations between Büzgülü grape ecotypes (Vitis vinifera L.) grown in different regions of Turkey were investigated by RAPD analysis. Of 50 decamer primers tested, 20 were used in the study, and 17 yielded clear and reproducible polymorphic bands. Differences between ecotypes were analyzed statistically. The similarity index was lowest (0.601) between Etli Büzgülü (collection no. 619, from Antalya province) and Büzgülü (no. 572, Eskişehir prov.), and highest (0.879) between Sik Büzgülü (no. 516, Konya prov.) and Büzgülü (no. 738, Kütahya prov.). The data matrix indicated genetic distances ranging up to 0.038, although most were under 0.03. Phylogenetic trees obtained by UPGMA and neighbor-joining methods basically resembled each other, with some differences. The results from RAPD analysis correlated with some morphological characteristics. This study of Turkish grape germplasm suggests that the geographical and ecological distribution of the plants contributes to higher genetic similarity. 

Key Words: Vitis vinifera L., Büzgülü, polymorphism, RAPD, Turkey.