ACTA BIOLOGICA CRACOVIENSIA Series Botanica 47/2: 145-151, 2005

Influence of Cultivar, Explant Source and Plant Growth Regulator on Callus Induction and Plant Regeneration of Cannabis Sativa L.

Aurelia Ślusarkiewicz-Jarzina*, Aleksandra Ponitka, Zygmunt Kaczmarek

Institute of Plant Genetics, Polish Academy of Sciences,
ul. Strzeszyńska 34, 60–479 Poznań

*e-mail: ajar@igr.poznan.pl

Received March 28, 2005; revision accepted August 10, 2005

The effects of different combinations of plant growth regulators (PGRs) on callus induction and plant regeneration were investigated in five cultivars of Cannabis sativa L. Callus was induced from different explant sources (young leaves, petioles, internodes, axillary buds) on MS basal medium with various concentrations of PGRs (2,4-D, DICAMBA, KIN, NAA). The highest frequency of callus induction (avg. 82.7% of eight medium combinations) was exhibited by petiole explants of cv. Fibrimon-24. Plant regeneration was obtained from all studied cultivars. The highest number of plants was regenerated from callus tissue of petiole explants on MS medium containing DICAMBA. A total of 46 plants (1.35% of callus) were regenerated: 16 (0.47%) from cv. Silesia petioles, 7 (0.20%) from cv. Novosadska petioles, 6 (0.18%) from cv. Fedrina 74 petioles, 12 (0.35%) from cv. Fibrimon 24 axillary buds, and 5 (0.15%) from cv. Juso 15 internodes. Significant improvement of hemp plant regeneration in vitro was achieved.

Key words: Cannabis sativa, axillary buds, callus induction, plant growth regulators, internode, leaf, petiole, plant regeneration. 

Abbreviations: 2,4-D – dichlorophenoxyacetic acid; DICAMBA – 3,6-dichloro-o-anisic acid; KIN – 6-furfurylaminopurine; NAA – 1-naphthaleneacetic acid. Abbreviations: 2,4-D – dichlorophenoxyacetic acid; DICAMBA – 3,6-dichloro-o-anisic acid; KIN – 6-furfurylaminopurine; NAA – 1-naphthaleneacetic acid.